Immunohistochemical expression of ?-catenin, Ki67, CD3 and CD18 in canine colorectal adenomas and adenocarcinomas

Abstract Background Inflammation is believed to influence human colorectal carcinogenesis and may have an impact on prognosis survival. The mucosal immunophenotype in dogs with cancer poorly described. aim of this study was investigate whether the density, distribution grade tumor-infiltrating immune cells (TIIs) are different normal colonic tissue vs benign stages (adenomas) malignant (adenocarcinomas) canine carcinogenesis, thus, they can be considered as prognostic factors dogs. This retrospective case-control performed formalin-fixed, paraffin-embedded samples from histologically confirmed adenoma ( n = 18) adenocarcinoma 13) collected archived samples. had been by colonoscopy, surgery or during postmortem examination. Healthy obtained post mortem euthanized for reasons not involving gastrointestinal tract served control 9). Results tumor significantly lower numbers CD3+ T-cells epithelium compared controls (adenocarcinoma control, Kruskal-Wallis test, p 0.0004, 0.002). Adenomas a number CD18+ lamina propria, (Kruskal-Wallis 0.008). Colonic uniform staining ?-catenin along cell membrane epithelial cells. Compared cells, expression levels cytoplasmic were higher adenomas adenocarcinomas (adenoma 0.004, None showed positive nucleus In contrast, moderate strong nucleus. nuclear (signal strength distribution) < 0.05). Conclusions Ki67 useful markers demonstrating progression adenocarcinomas. presence CD18 tumors indicates reduced histiocytes T-cells, which implications pathogenesis